New PDF release: Advances in Clinical Chemistry, Vol. 32

By Herbert E. Spiegel

ISBN-10: 012010332X

ISBN-13: 9780120103324

Compliment for the sequence: "Its continuity of pertinence, excellence, and authority is still unbroken - a tribute to the skilful enhancing and writing concerned. each educated laboratory employees should have to be had a duplicate of this volume." --Clinical Chemistry For greater than thirty years, this serial has broadened the technical scope and accelerated the clinical base of scientific chemistry. those volumes make clear the components of molecular biology, informatics, and the tracking of physiological parameters in severe events as they pertain to medical chemistry. each one quantity of Advances in scientific Chemistry comprises an index, and every bankruptcy comprises references.

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3. 4. 5. 6. 7. 8. 39 43 45 46 47 48 50 50 52 53 54 54 55 56 57 58 58 68 69 70 70 1. S. Food and Drug Administration. 39 Copynghi 0 1996 by Academic Press, Inc All nzhts of re~roductiontn anv form reserved 40 KAISER J. AZIZ (hematology, microbiology, virology, tissue typing) and that have applications for genetic disorders, oncology, transplantation, and infectious diseases. Examples are nucleic acid hybridization (dot blot, Southern blot), in situ hybridization, and in vitro amplification technologies such as the polymerase chain reaction (PCR).

These techniques produce alternating patterns of light and dark bands that are characteristic for each chromosome. Two types of banding techniques are used. These are fluorescent quinacrine or Q banding, and Giemsa or G banding. These banding techniques produce similar patterns except for the brilliant fluorescence of the Y chromosome in the Q banding technique. Other alternative techniques, called R and T banding, utilize heat denaturation, and their staining intensity is inversely related to staining found in Q or G banding, to the extent that areas that stain lightly in G or Q banding stain intensely in R and T banding, and vice versa.

The ability to coat paramagnetic beads with antibodies to surface antigens of bacteria has been exploited to separate and concentrate organisms from the sample. The bacteria can then be lysed, and the DNA released into the supernatant can be amplified by PCR. Such an approach has found a wide range of applications, among which is the detection of Helicobacter pylori in water and stool specimens (El). Identification of pathogenic fungi belonging to the genus Candida has been made possible by using nested PCR to amplify first the entire small subunit (ssu) rRNA gene and subsequently a variable region within this gene.

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Advances in Clinical Chemistry, Vol. 32 by Herbert E. Spiegel


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